The hippoboscids play important roles in ecosystem functioning and can serve as indicators of biodiversity and ecosystem health. These bloodsucking ectoparasites are important from a public health perspective, as they can serve as vectors of various endoparasitic pathogens in animals and humans, although our understanding is still limited in this case. The current study provides information on the host species and distribution, which can contribute to the understanding of the ecology and epidemiology of these important arthropods with a potential impact on both animal and human health. We present an ecological parasite-host interaction network of louse flies and deer keds (Diptera: Hippoboscidae) collected from various sites in Slovakia from this time. A total of 123 new samples of eight hippoboscid fly species were captured on 17 species, including 15 birds and two mammals. New host-parasite interactions were recorded for the first time.