Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats

Abstract

This study was aimed to investigate the effects of diosmin (DIO) in rats exposed to emamectin benzoate (EB). For this purpose, a total of 60 Wistar Albino male rats, aged 6 to 8 weeks and weighing 180 to 250 g, were used, 10 in each group. The groups were determined, respectively, control, EB (10 mg kg-1), DIO (50 mg kg-1), DIO (100 mg kg-1), EB (10 mg kg-1) + DIO (50 mg kg-1), EB (10 mg kg-1) + DIO (100 mg kg-1), and the indicated doses were applied by gavage for 21 days. At the end of the trial period, a heart puncture was performed under anesthesia and blood samples were taken into tubes with heparinized and anticoagulant-free properties. The liver, kidney, brain, testis, heart and lung tissues were removed after cervical dislocation. While some biochemical markers, such as aspartate aminotrans- ferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), triglyceride, cholesterol, total protein, albumin, uric acid, blood urea nitrogen (BUN) and, creatinine were assessed in the serum, oxidative stress indicators, such as MDA, NO, SOD, CAT, GSH-Px, GR, GST, and GSH, were determined in tissue samples. At the end of the investigation, no changes between the parameters of the DIO groups and the control group were discovered. Comparing the EB group to the control group, it was found that the levels of MDA and NO in tissues/ erythrocytes had significantly increased. The activity of the GSH, GR, GST, SOD, and CAT enzymes, as well as GSH levels, was found to have decreased. Furthermore, serum total protein and albumin levels were reduced, although AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levels/activities were elevated. Depend- ing on the dose, it was found that the values of the groups coadministered with EMB and DIO were close to those of the control group. In conclusion, it is proposed that DIO could provide protection against EMB-induced toxicity in rats.