The study of acute toxicity and some clinical parameters of a new chelated compound

Abstract

The parameters of acute toxicity of a chelate complex containing microelements: iron, manganese, copper, selenium, iodine was studied on outbred male rats. Toxic doses were determined by constructing a direct regression graph. Thus, groups of animals were formed that were administered an aqueous solution of the chelate complex in doses, ml/kg: 6.94; 8.89; 11.11 and 11.67. The response to the solution was assessed using generally accepted clinical and hematological methods. At the same time, the dynamics of temperature, live weight, mortality and peripheral blood parameters were taken into account. During the experiment, toxicity indicators were determined. The toxic effect in experimental animals manifested itself in the form of inhibition of activity, shortness of breath, decreased reactions to external stimuli, decreased body temperature, ruffled coat, and weight loss. In the blood, a significant increase in the number of leukocytes and erythrocytes was noted in rats receiving subsidies of the chelate microelement complex in doses of 8.89 ml/kg and 11.11 ml/kg. It was concluded that the introduction of a chelate complex of microelements led to stimulation of erythropoiesis and leukopoiesis, and according to the results of a study of toxicity indicators, the presented formulation corresponds to the 3rd hazard class. The parameters of acute toxicity of the chelate complex of microelements were determined, taking into account the survival of animals, LD50 was established - 907 ± 0.63 mg/kg; LD16 – 765 mg/kg; LD84 – 1076 mg/kg. It was concluded that the toxicity indicators of the chelate complex of microelements according to GOST 12.1.007–76 correspond to the 3rd hazard class - “moderately hazardous substances”. Considering the potential hematopoietic and antioxidant effect of trace elements in the chelate complex and the relatively low toxicity with other seleniumcontaining organic and inorganic additives and preparations, the use of this complex opens up opportunities and prospects for testing as a radioprotective agent.