Effects of Hybrid Susceptibility and Inoculation Timing on Goss’s Bacterial Wilt and Leaf Blight Severity and Corn Yield

Abstract

Goss’s bacterial wilt and leaf blight (Goss’s wilt) of corn is the most important corn disease in North Dakota (ND), and yield loss due to the disease has not been reliably quantified in northern corn growing regions. To help quantify the amount of yield loss caused by Goss’s wilt, a total of six field experiments were conducted from 2015 to 2017. Experiments were designed in a randomized complete block with a split plot arrangement. Hybrids served as main plots and Clavibacter nebraskensis inoculation timings as subplots. Three hybrids were used and classified as susceptible, moderately susceptible, and resistant. Inoculation timings included a noninoculated control, six to 10 leaf collars (V6 to V10), reproductive silk stage (R1), or a sequential combination of V6 to V10 and R1. A high level of disease (greater than 50% on susceptible hybrid) occurred in three experiments, a low level of disease (less than 5% on susceptible hybrid) in one experiment, and no disease was reported in two experiments. A combined analysis of the high disease experiments indicated yield losses of 34 to 41% on the susceptible hybrid when C. nebraskensis inoculation occurred at V6 to V10. Yield losses of 22 to 25% occurred on the moderately susceptible hybrid when C. nebraskensis inoculation occurred at V6 to V10, and statistical differences in yield loss were not found among inoculations timings on the resistant hybrid. Correlation analyses suggest that for every 1% increase in R1 disease severity on the susceptible hybrid, yield was reduced by 117 kg/ha (1.9 bu/acre). The current study further demonstrates the importance of hybrid resistance and provides updated yield loss information on Goss’s wilt in a northern corn growing region.