Antioxidant Activity of Entrapped alfa-tocopherol and Lutein in PLGA Nanoparticles in Wistar Rats

Abstract

The hypercaloric diet leads to obesity which is a risk factor for developing many systemic diseases such as diabetes, cardiovascular diseases and cancer. The purpose of our study was to observe the antioxidants effects of entrapped alfa-tocopherol (vitamin E) and lutein in Poly Lactic-CO-Glycolic acid (PLGA) in Wistar rats that received 3 weeks hypercaloric diet. Hepatic and splenic lysates have been used to detect changes in total antioxidant capacity (TAC) and advanced oxidative protein products (AOPP). The AOPP liver levels were statistically decreased in the case of rats groups that received a daily dose of PLGA-lutein or PLGA-vitamin E versus the control group. The AOPP level was decreased but statistically insignificant in Wistar rats spleen who received a daily dose of PLGA-lutein. At hepatic level, TAC was statistically increased in rats groups that received a daily dose of vitamin E or lutein. An increased statistical level of TAC was observed in the spleen cell lysing in Wistar rats who received the daily dose of PLGA-vitamin E. Entrapment of vitamin E and lutein in PLGA structure has diminished the effects of hypercaloric diet and can be considered a attractive and promising approach to enhance the bioavailability and activity of poorly water soluble compounds.