Author:
Bayatinejad Ghazal,Salehi Mohammadreza,Beigverdi Reza,Halimi Shahnaz,Emaneini Mohammad,Jabalameli Fereshteh
Abstract
Abstract
Background
Hospital infections such as ventilator-associated pneumonia (VAP) due to multidrug-resistant Klebsiella pneumoniae (MDR-KP) strains have increased worldwide. In addition, biofilm production by these resistant isolates has confronted clinicians with higher treatment failure and infection recurrence. Given the paucity of new agents and limited data on combination therapy for MDR-KPs, the present study sought to evaluate the in vitro activity of several antibiotic combinations against planktonic and biofilm MDR-KPs isolated from patients with VAP.
Results
All 10 carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates demonstrated multidrug resistance against the tested antibiotics. At planktonic mode, combinations of colistin-meropenem and amoxicillin/clavulanate in combination with meropenem, colistin, or amikacin showed synergism against 60–70% isolates. On the other hand, in the biofilm state, colistin-based combinations exhibited synergism against 50–70% isolates and the most effective combination was colistin-amikacin with 70% synergy.
Conclusions
The results revealed that combinations of amoxicillin/clavulanate with colistin, meropenem, or amikacin in the planktonic mode and colistin with amoxicillin/clavulanate, meropenem, or amikacin in the biofilm mode could effectively inhibit CRKP isolates, and thus could be further explored for the treatment of CRKPs.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference49 articles.
1. Kalil AC, Metersky ML, Klompas M, Muscedere J, Sweeney DA, Palmer LB, et al. Management of adults with hospital-acquired and ventilator-associated pneumonia: 2016 clinical practice guidelines by the infectious Diseases Society of America and the american thoracic society. Clin Infect Dis. 2016;63(5):e61–e111.
2. Moradi M, Nili F, Nayeri F, Amini E, Esmaeilnia T. Study of characteristics, risk factors and outcome for Ventilator Associated Pneumonia in neonatal intensive care unit patient. Tehran Univ Med J. 2013;71(6).
3. Kalanuria AA, Mirski M, Ziai W. Ventilator-associated pneumonia in the ICU. Annual Update in Intensive Care and Emergency Medicine. 2014;2014:65–77.
4. Selina F, Talha KA, Islam A, Hasan Z, Hyder M, Selvapandian S. Organisms associated with ventilator associated pneumonia (VAP) in intensive care units (ICU). J Bangladesh Soc Anaesthesiologists. 2009;22(2):72–7.
5. Gil-Perotin S, Ramirez P, Marti V, Sahuquillo JM, Gonzalez E, Calleja I, et al. Implications of endotracheal tube biofilm in ventilator-associated pneumonia response: a state of concept. Crit Care. 2012;16(3):1–9.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献