Machine learning and phylogenetic analysis allow for predicting antibiotic resistance in M. tuberculosis

Author:

Yurtseven Alper,Buyanova Sofia,Agrawal Amay Ajaykumar,Bochkareva Olga O.,Kalinina Olga V.

Abstract

Abstract Background Antimicrobial resistance (AMR) poses a significant global health threat, and an accurate prediction of bacterial resistance patterns is critical for effective treatment and control strategies. In recent years, machine learning (ML) approaches have emerged as powerful tools for analyzing large-scale bacterial AMR data. However, ML methods often ignore evolutionary relationships among bacterial strains, which can greatly impact performance of the ML methods, especially if resistance-associated features are attempted to be detected. Genome-wide association studies (GWAS) methods like linear mixed models accounts for the evolutionary relationships in bacteria, but they uncover only highly significant variants which have already been reported in literature. Results In this work, we introduce a novel phylogeny-related parallelism score (PRPS), which measures whether a certain feature is correlated with the population structure of a set of samples. We demonstrate that PRPS can be used, in combination with SVM- and random forest-based models, to reduce the number of features in the analysis, while simultaneously increasing models’ performance. We applied our pipeline to publicly available AMR data from PATRIC database for Mycobacterium tuberculosis against six common antibiotics. Conclusions Using our pipeline, we re-discovered known resistance-associated mutations as well as new candidate mutations which can be related to resistance and not previously reported in the literature. We demonstrated that taking into account phylogenetic relationships not only improves the model performance, but also yields more biologically relevant predicted most contributing resistance markers.

Funder

Klaus Faber Foundation

HelmholtzAI project AMR-XAI

Fonds zur Förderung der Wissenschaftlichen Forschung (FWF), Grant ESP 253-B

Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)

Publisher

Springer Science and Business Media LLC

Subject

Microbiology (medical),Microbiology

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