Abstract
Abstract
Background
The K1, K2, and K28 toxins are usually encoded by several cytoplasmically genetic satellite dsRNAs (M1, M2, and M28), which are encapsulated with virus-like particles (VLPs) and reliant on an additional assembly of assistant yeast viruses (L-A) for their reproduction and encapsidation. Ascomycetous yeast species that have these VLPs are especially attractive targets for finding killer toxins like proteins. This is because the organisms are known in producing a large variety of secondary metabolites and extracellular enzymes, which have medical importance as alternative drugs for resistance bacterial strains, particularly multi-resistance drugs (MRD).
Results
For the first time, 31 type strains of yeasts were tested for killer toxin production in Iraq via the measurement of gene expression of three killer toxin genes (M1, M2, and M28) within the mycovirus in yeasts. All the type strains gave an expression for the three killer toxins with variable levels. The highest expression was recorded for the killer toxin genes in Torulaspora delbrueckii followed by Wickerhamomyces anomalus. Determined antibacterial activity of two killer toxins appeared with high inhibition zone against pathogenic strains of bacteria. Cytotoxicity against human blood cells was not found. These results considered the first record of killer toxins isolated from type strains in Iraq.
Conclusion
The two typical strains Torulaspora delbrueckii and Wickerhamomyces anomalus showed the highest level of gene expression for the three killer toxins.
Publisher
Springer Science and Business Media LLC
Reference26 articles.
1. Abu-Mejdad NM (2019). Isolation and molecular identification of yeasts, study their potential for producing killer toxins and evaluation of toxins activity against some bacteria and pathogenic fungi. Basrah: PhD thesis.: 1-249
2. Ahmed A, Sesti F, Ilan N, Shih TM, Sturley SL, Goldstein SA (1999) A molecular target for viral killer toxin: TOK1 potassium channels. Cell. 99(3):283–291
3. Al-Qaysi SA, Al-Haideri H, Thabit ZA, Al-Kubaisy WHA, Ibrahim JA (2017) Production, characterization, and antimicrobial activity of mycocin produced by Debaryomyces hansenii DSMZ70238. International journal of microbiology 2017
4. Becker B, Schmitt M (2017) Yeast killer toxin K28: biology and unique strategy of host cell intoxication and killing. Toxins. 9(10):333
5. Buzzini P, Turchetti B, Vaughan-Martini AE (2007) The use of killer sensitivity patterns for biotyping yeast strains: the state of the art, potentialities and limitations. FEMS Yeast Res 7(6):749–760
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