Abstract
Abstract
Background
Chitosan is a polycationic polysaccharide derived from chitin that has been recognized as an effective elicitor in the production of secondary metabolites of many medicinal plants. In this study, the effect of abiotic elicitor (chitosan) at various concentrations on rosmarinic acid (RA) and total phenolic accumulation in shoot cultures of lemon balm was investigated.
Results
Treatment of shoots by chitosan led to a noticeable induction of phenylalanine ammonia-lyase (PAL), catalase (CAT), guaiacol peroxidase (GPX) and lipoxygenase (LOX) activities. Besides, the expression of PAL1, TAT and RAS genes and accumulation of RA and phenolic compound increased in chitosan-treated lemon balm shoots. Chitosan treatment also increased H2O2 accumulation and the expression of RBOH, an essential gene implicated in ROS production. Also, the up-regulation of the OPR gene by exogenous chitosan was associated with the induction of endogenous JA determined by GC-MASS.
Conclusion
The present study showed that the induced production of rosmarinic acid by chitosan involves the trigger of defense-related enzymes, up-regulated expression of TAT and RAS genes, and stimulation of JA biosynthesis.
Publisher
Springer Science and Business Media LLC
Reference66 articles.
1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
2. Agrawal GK, Rakwal R, Tamogami S, Yonekura M, Kubo A, Saji H (2002) Chitosan activates defense/stress response(s) in the leaves of Oryza sativa seedlings. Plant Physiol Biochem 40:1061–1069
3. Alami I, Jouy N, Clervet A (1999) The lipoxygenase pathway is involved in elicitor-induced phytoalexin accumulation in plane tree (Platanus acerifolia) cell-suspension cultures. J Phytopathol 147:515–519
4. Alexieva V, Sergiev I, Mapelli S, Karanov E (2001) The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ 24:1337–1344
5. Algam S, Xie G, Li B, Yu S, Su T, Larsen J (2010) Effects of Paenibacillus strains and chitosan on plant growth promotion and control of Ralstonia wilt in tomato. J. Plant Pathol 92:593–600
Cited by
39 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献