Author:
Liu Nian,Gujrati Vipul,Werner Juan Pablo Fuenzalida,Mishra Kanuj,Anzenhofer Pia,Stiel Andre C.,Mettenleiter Gabriele,Feuchtinger Annette,Walch Axel,Ntziachristos Vasilis
Abstract
Abstract
Background
Cationic dyes are widely used as biomarkers for optical imaging. However, most of these are hydrophobic and cannot be employed in vivo without chemical conjugation or modification. Herein, we report for the first time the use of bacterial outer membrane vesicles (OMVs) as nanocarriers of cationic dyes for cancer theranostics.
Results
We demonstrate that cationic dyes (IR780, Cy7, and Cy7.5) form stable complexes with negatively charged bacterial-OMVs, improving the dyes’ in vivo circulation and optoacoustic properties. Such OMV-Dye complexes are biodegradable and safe for in vivo applications. Importantly, this method of cationic dye loading is faster and easier than synthetic chemistry approaches, and the efficient tumor accumulation of OMV-Dyes enables sensitive tumor detection using optoacoustic technology. As a proof-of-concept, we generated OMV-IR780 for optoacoustics-guided in vivo tumor phototherapy in a mouse model.
Conclusions
Our results demonstrate cationic dye-bound OMVs as promising novel nanoagents for tumor theranostics.
Funder
Technische Universität München
Publisher
Springer Science and Business Media LLC
Subject
Physical and Theoretical Chemistry,Pharmaceutical Science,Oncology,Biomedical Engineering
Cited by
2 articles.
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