Author:
Wang Bingbing,Tan Min,Li Wei,Xu Qinghua,Jin Lianfeng,Xie Shuanshuan,Wang Changhui
Abstract
Abstract
Background
Community-acquired pneumonia (CAP) patients with chronic obstructive pulmonary disease (COPD) have higher disease severity and mortality compared to those without COPD. However, deep investigation into microbiome distribution of lower respiratory tract of CAP with or without COPD was unknown.
Methods
So we used metagenomic next generation sequencing (mNGS) to explore the microbiome differences between the two groups.
Results
Thirty-six CAP without COPD and 11 CAP with COPD cases were retrieved. Bronchoalveolar lavage fluid (BALF) was collected and analyzed using untargeted mNGS and bioinformatic analysis. mNGS revealed that CAP with COPD group was abundant with Streptococcus, Prevotella, Bordetella at genus level and Cutibacterium acnes, Rothia mucilaginosa, Bordetella genomosp. 6 at species level. While CAP without COPD group was abundant with Ralstonia, Prevotella, Streptococcus at genus level and Ralstonia pickettii, Rothia mucilaginosa, Prevotella melaninogenica at species level. Meanwhile, both alpha and beta microbiome diversity was similar between groups. Linear discriminant analysis found that pa-raburkholderia, corynebacterium tuberculostearicum and staphylococcus hominis were more enriched in CAP without COPD group while the abundance of streptococcus intermedius, streptococcus constellatus, streptococcus milleri, fusarium was higher in CAP with COPD group.
Conclusions
These findings revealed that concomitant COPD have an mild impact on lower airway microbiome of CAP patients.
Funder
Shanghai Science and Technology
Publisher
Springer Science and Business Media LLC
Reference29 articles.
1. Gadsby NJ, Musher DM. The microbial etiology of community-acquired pneumonia in adults: from classical bacteriology to host transcriptional signatures. Clin Microbiol Rev. 2022;35(4):e0001522.
2. Niederman MS, Torres A. Severe community-acquired pneumonia. Eur Respir Rev. 2022;31(166).
3. Jiang N, Long QY, Zheng YL, Gao ZC. Advances in epidemiology, etiology, and treatment of community-acquired pneumonia. Zhonghua yu fang yi xue za zhi. 2023;57(1):91–9.
4. Hsu CW, Suk CW, Hsu YP, Chang JH, Liu CT, Huang SK, Hsu SC. Sphingosine-1-phosphate and CRP as potential combination biomarkers in discrimination of COPD with community-acquired pneumonia and acute exacerbation of COPD. Respir Res. 2022;23(1):63.
5. Yang J, Zhang Q, Zhang J, Ouyang Y, Sun Z, Liu X, Qaio F, Xu LQ, Niu Y, Li J. Exploring the change of host and microorganism in chronic obstructive pulmonary disease patients based on metagenomic and metatranscriptomic sequencing. Front Microbiol. 2022;13:818281.
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