Evaluation of nephroprotective and cytotoxic effect of ethanolic extract of Mikania scandens leaves by using alloxan-induced diabetic nephropathy mice

Abstract

Abstract Background Diabetic is one of the fundamental persuasive of diabetic nephropathy and significantly sparks off kidney diseases and end stage renal failure globally. Method The current research was carried out to evaluate hypoglycemic potential and nephroprotective effect of ethanolic extract of Mikania scandens leaves by using alloxan-induced diabetic nephropathy (DN) mice. The mice were intraperitoneally treated with (200 mg/kg) Mikania scandens leaves extract and standard (120 mg/kg) metformin HCL solution up to 22 days. During this treatment period, we collected blood for evaluation of different types of parameters such as blood glucose level body weight at 0, 15, 22th days, serum creatinine, uric acid, total protein were estimated at the end of the experiment (day 22). Result Mikania scandens leaves extract significantly (p < 0.05) lessen blood glucose level, serum creatinine, total protein and uric acid. Apart this, cytotoxicity studies were assessed by Brine Shrimp Lethality Bioassay. By this method, we measure the dose of LC50. The plant has high LC50 dose thus cytotoxicity has ensued at massive amount and safe to administer. Conclusion Lowering of serum creatinine, uric acid and total protein recommend that the ethanolic extract of Mikania scandens possess potent nephroprotective activity and assure the traditional avail of this plant in the management of diabetes nephropathy.