Author:
Sun Ting,Pei Shengwei,Liu Yangkai,Hanif Quratulain,Xu Haiyue,Chen Ningbo,Lei Chuzhao,Yue Xiangpeng
Abstract
Abstract
Backgroud
The single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) are two major genomic variants, which play crucial roles in evolutionary and phenotypic diversity.
Results
In this study, we performed a comprehensive analysis to explore the genetic variations (SNPs and CNVs) of high sperm motility (HSM) and poor sperm motility (PSM) Simmental bulls using the high-coverage (25×) short-read next generation sequencing and single-molecule long reads sequencing data. A total of ~ 15 million SNPs and 2,944 CNV regions (CNVRs) were detected in Simmental bulls, and a set of positive selected genes (PSGs) and CNVRs were found to be overlapped with quantitative trait loci (QTLs) involving immunity, muscle development, reproduction, etc. In addition, we detected two new variants in LEPR, which may be related to the artificial breeding to improve important economic traits. Moreover, a set of genes and pathways functionally related to male fertility were identified. Remarkably, a CNV on SPAG16 (chr2:101,427,468 − 101,429,883) was completely deleted in all poor sperm motility (PSM) bulls and half of the bulls in high sperm motility (HSM), which may play a crucial role in the bull-fertility.
Conclusions
In conclusion, this study provides a valuable genetic variation resource for the cattle breeding and selection programs.
Funder
National natural science foundation of China
China Postdoctoral Science Foundation
the National Beef Cattle and Yak Industrial Technology System
Publisher
Springer Science and Business Media LLC
Cited by
2 articles.
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