Author:
Wei Wen-Yu,Gong Yi,Guo Xin-Fen,Liu Min,Zhou Yu-Lin,Li Zhi,Zhou Li,Wang Zhong-Wei,Gui Jian-Fang
Abstract
Abstract
Background
Red-tail catfish (Hemibagrus wyckioides) is an important commercially farmed catfish in southern China. Males of red-tail catfish grow faster than females, suggesting that all-male catfish will produce more significant economic benefits in aquaculture practice. However, little research has been reported on sex determination and gonadal development in red-tail catfish.
Results
In this study, we performed the first transcriptomic analysis of male and female gonads at four developmental stages at 10, 18, 30, and 48 days post hatching (dph) using RNA-seq technology. A total of 23,588 genes were screened in 24 sequenced samples, of which 28, 213, 636, and 1381 differentially expressed genes (DEGs) were detected at four developmental stages, respectively. Seven candidate genes of sex determination and differentiation were further identified. Real-time quantitative PCR (RT-qPCR) further confirmed that anti-Mullerian hormone (amh), growth differentiation factor 6a (gdf6a), testis-specific gene antigen 10 (tsga10), and cytochrome P450 family 17 subfamily A (cyp17a) were highly expressed mainly in the male, while cytochrome P450 family 19 subfamily A polypeptide 1b (cyp19a1b), forkhead box L2 (foxl2), and hydroxysteroid 17-beta dehydrogenase 1 (hsd17b1) were highly expressed in the female. The KEGG pathway enrichment data showed that these identified DEGs were mainly involved in steroid hormone biosynthesis and TGF-β signaling pathways.
Conclusions
Based on RNA-seq data of gonads at the early developmental stages, seven DEGs shared by the four developmental stages were identified, among which amh and gdf6a may be the male-biased expression genes, while foxl2, cyp19a1b and hsd17b1 may be the female-biased expression genes in red-tail catfish. Our study will provide crucial genetic information for the research on sex control in red-tail catfish, as well as for exploring the evolutionary processes of sex determination mechanisms in fish.
Funder
National Natural Science Foundation of China
National Key Research and Development Program of China
Publisher
Springer Science and Business Media LLC
Reference100 articles.
1. Hee NH, Rainboth WJ. The Bagrid catfish Genus Hemibagrus (Teleostei : Siluriformes) in central Indochina with a new species from the Mekong River. Raffles B Zool. 1999;47(2):555–76.
2. Xue CJ, Tian SK, Li YM, Li XS, Liu YT, Leng Y, Zhang JS, He WP. Preliminary report on the domestication and artificial propagation of Mystus wyckioides (in Chinese). J Hydroecol. 2010;31(4):142–5.
3. Zhang YJ, Liu ZY, Song WH, Wang X. The biology of Mystus wyckioides and its status of introduction and culture (in Chinese). J Hydroecol. 2007;27(5):41–2.
4. Zhou YL, Wu JJ, Wang ZW, Li GH, Zhou L, Gui JF. Microsatellite polymorphism and genetic differentiation of different populations screened from genome survey sequencing in red-tail catfish (Hemibagrus wyckioides). Aquacult Rep. 2021;19:100614.
5. Wang D, Mao HL, Chen HX, Liu HQ, Gui JF. Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations. Anim Genet. 2009;40(6):978–81.
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献