Author:
Wang Xiao-Li,Zhang Ru-Nan,Pan Yu-Lin,Li Zhi-Ming,Li Hong-Qiu,Lei Ya-Ting,Zhao Fang-Fang,Hao Xiao-Xiao,Ma Wei-Wei,Yu Cui-Ping,Yao Hong-Wei,Wang Xin-Yu,Lv Jun-Jie,Wu Yong-Hui,Wang Sheng-Yuan
Abstract
Abstract
Background
The impact of acrylamide (ACR) on learning and memory has garnered considerable attention. However, the targets and mechanisms are still unclear.
Results
Elongation factor 2 (eEF2) was significantly upregulated in the results of serum proteomics. Results from in vitro and in vivo experiments indicated a notable upregulation of Eukaryotic elongation factor 2 kinase (eEF2K), the sole kinase responsible for eEF2 phosphorylation, following exposure to ACR (P < 0.05). Subsequent in vitro experiments using eEF2K siRNA and in vivo experiments with eEF2K-knockout mice demonstrated significant improvements in abnormal indicators related to ACR-induced learning and memory deficits (P < 0.05). Proteomic analysis of the hippocampus revealed Lpcat1 as a crucial downstream protein regulated by eEF2K. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses indicated that eEF2K may play a role in the process of ACR-induced learning and memory impairment by affecting ether lipid metabolism.
Conclusions
In summary, eEF2K as a pivotal treatment target in the mechanisms underlying ACR-induced learning and memory impairment, and studies have shown that it provides robust evidence for potential clinical interventions targeting ACR-induced impairments.
Funder
National Natural Science Foundation of China
National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
Special funding for postdoctoral fellows in Heilongjiang Province
Publisher
Springer Science and Business Media LLC