Author:
Bhattarai Rudra,Liu Hui,Siddique Kadambot H.M.,Yan Guijun
Abstract
AbstractBackgroundWeeds reduce wheat yields in dryland farming systems. Herbicides such as metribuzin are commonly used to control weeds. However, wheat has a narrow safety margin against metribuzin. Standing crops such as wheat with weeds in the same field can also be killed by the same dose of metribuzin. Therefore, it is important to identify metribuzin resistance genes and understand the resistance mechanism in wheat for sustainable crop production. A previous study identified a significant metribuzin resistance wheat QTL,Qsns.uwa.4 A.2, explaining 69% of the phenotypic variance for metribuzin resistance.ResultsTwo NIL pairs with the most contrasting performance in the metribuzin treatment and different in genetic backgrounds were compared using RNA sequence analysis, identifying nine candidate genes underlyingQsns.uwa.4 A.2responsible for metribuzin resistance. Quantitative RT-qPCR further validated the candidate genes, withTraesCS4A03G1099000(nitrate excretion transporter),TraesCS4A03G1181300(aspartyl protease), andTraesCS4A03G0741300(glycine-rich proteins) identified as key factors for metribuzin resistance.ConclusionIdentified markers and key candidate genes can be used for selecting metribuzin resistance in wheat.
Funder
Department of Industry, Science, Energy and Resources, Australian Government
Publisher
Springer Science and Business Media LLC
Reference79 articles.
1. Roberts J, Florentine S. Biology, distribution and control of the invasive species Ulex europaeus (Gorse): a global synthesis of current and future management challenges and research gaps. Weed Res. 2021;61(4):272–81.
2. Ryan J, De Pauw E, Gomez H, Mrabet R. Drylands of the Mediterranean zone: biophysical resources and cropping systems. Dryland Agric. 2006;23:577–624.
3. Rose TJ, Parvin S, Han E, Condon J, Flohr BM, Schefe C, et al. Prospects for summer cover crops in southern australian semi-arid cropping systems. Agric Syst. 2022;200:103415.
4. Kraehmer H, Laber B, Rosinger C, Schulz A. Herbicides as weed control agents: state of the art: I. weed control research and safener technology: the path to modern agriculture. Plant Physiol. 2014;166(3):1119–31.
5. Khalil Y. Interaction of pre-emergent herbicides and crop residues in western australian no-tillage systems. The University of Western Australia; 2018.