Author:
Ajayi Oyeyemi O.,Bregitzer Phil,Klos Kathy,Hu Gongshe,Walling Jason G.,Mahalingam Ramamurthy
Abstract
Abstract
Background
With ongoing climate change, drought events are severely limiting barley production worldwide and pose a significant risk to the malting, brewing and food industry. The genetic diversity inherent in the barley germplasm offers an important resource to develop stress resiliency. The purpose of this study was to identify novel, stable, and adaptive Quantitative Trait Loci (QTL), and candidate genes associated with drought tolerance. A recombinant inbred line (RIL) population (n = 192) developed from a cross between the drought tolerant ‘Otis’ barley variety, and susceptible ‘Golden Promise’(GP) was subjected to short-term progressive drought during heading in the biotron. This population was also evaluated under irrigated and rainfed conditions in the field for yields and seed protein content.
Results
Barley 50k iSelect SNP Array was used to genotype the RIL population to elucidate drought-adaptive QTL. Twenty-three QTL (eleven for seed weight, eight for shoot dry weight and four for protein content) were identified across several barley chromosomes. QTL analysis identified genomic regions on chromosome 2 and 5 H that appear to be stable across both environments and accounted for nearly 60% variation in shoot weight and 17.6% variation in seed protein content. QTL at approximately 29 Mbp on chromosome 2 H and 488 Mbp on chromosome 5 H are in very close proximity to ascorbate peroxidase (APX) and in the coding sequence of the Dirigent (DIR) gene, respectively. Both APX and DIR are well-known key players in abiotic stress tolerance in several plants. In the quest to identify key recombinants with improved tolerance to drought (like Otis) and good malting profiles (like GP), five drought tolerant RILs were selected for malt quality analysis. The selected drought tolerant RILs exhibited one or more traits that were outside the realms of the suggested limits for acceptable commercial malting quality.
Conclusions
The candidate genes can be used for marker assisted selection and/or genetic manipulation to develop barley cultivars with improved tolerance to drought. RILs with genetic network reshuffling necessary to generate drought tolerance of Otis and favorable malting quality attributes of GP may be realized by screening a larger population.
Funder
Agricultural Research Service
Publisher
Springer Science and Business Media LLC
Reference77 articles.
1. Folger P. Drought in the United States: causes and current understanding. Congressional Res Service. 2017.
2. United Nations. : Department of Economic and Social Affairs - Population Division:. 2019.
3. Gosal SS, Wani SH, Kang MS. Biotechnology and Drought Tolerance. J Crop Improv. 2009;23(1):19–54.
4. Kadam NN, Xiao G, Melgar RJ, Bahuguna RN, Quinones C, Tamilselvan A, Prasad PVV, Jagadish KSV. Agronomic and physiological responses to high temperature, Drought, and elevated CO2 interactions in Cereals. In. 2014;127:111–56.
5. Wang Q, Wu J, Lei T, He B, Wu Z, Liu M, Mo X, Geng G, Li X, Zhou H, et al. Temporal-spatial characteristics of severe drought events and their impact on agriculture on a global scale. Quatern Int. 2014;349:10–21.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献