Author:
Chang Jingjing,Li Jiawei,Li Jinlong,Chen Xiao,Jiao Jiabin,Li Jing,Song Zhao,Zhang Baige
Abstract
Abstract
Background
Hydrogen gas (H2), a novel and beneficial gaseous molecule, plays a significant role in plant growth and development processes. Hydrogen-rich water (HRW) is regarded as a safe and easily available way to study the physiological effects of H2 on plants. Several recent research has shown that HRW attenuates stress-induced seed germination inhibition; however, the underlying modes of HRW on seed germination remain obscure under non-stress condition.
Results
In this current study, we investigated the possible roles of gibberellin (GA) and abscisic acid (ABA) in HRW-regulated seed germination in wax gourd (Benincasa hispida) through pharmacological, physiological, and transcriptome approaches. The results showed that HRW application at an optimal dose (50% HRW) significantly promoted seed germination and shortened the average germination time (AGT). Subsequent results suggested that 50% HRW treatment stimulated GA production by regulating GA biosynthesis genes (BhiGA3ox, BhiGA2ox, and BhiKAO), whereas it had no effect on the content of ABA and the expression of its biosynthesis (BhiNCED6) and catabolism genes (BhiCYP707A2) but decreased the expression of ABA receptor gene (BhiPYL). In addition, inhibition of GA production by paclobutrazol (PAC) could block the HRW-mediated germination. Treatment with ABA could hinder HRW-mediated seed germination and the ABA biosynthesis inhibitor sodium tungstate (ST) could recover the function of HRW. Furthermore, RNA-seq analysis revealed that, in the presence of GA or ABA, an abundance of genes involved in GA, ABA, and ethylene signal sensing and transduction might involve in HRW-regulated germination.
Conclusions
This study portrays insights into the mechanism of HRW-mediated seed germination, suggesting that HRW can regulate the balance between GA and ABA to mediate seed germination through ethylene signals in wax gourd.
Funder
Science and Technology Talent Introduction Special Fund Project of Guangdong Academy of Agricultural Sciences
Guangdong Province Modern Agriculture Industry Technology System Innovation Team Construction Project
Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences
the Foshan Special Fund-Construction Project of High-level Agricultural Science and Technology Demonstration City of GDAAS in 2021
Publisher
Springer Science and Business Media LLC
Reference56 articles.
1. Gu M, Fan S, Liu G, Guo L, Ding X, Lu Y, et al. Extract of wax gourd peel prevents high-fat diet-induced hyperlipidemia in C57BL/6 mice via the inhibition of the PPARγ pathway. Evid Based Compl Alt Med. 2013;2013:342561.
2. Zhang B, Cakmak I, Feng J, Yu C, Chen X, Xie D, et al. Magnesium deficiency reduced the yield and seed germination in wax gourd by affecting the carbohydrate translocation. Front Plant Sci. 2020;11:797.
3. Sharma LK. Food Medicines. Pedukkottai: Natural Cure Publishing House; 1984.
4. Jiang B, Liu W, Xie D, Peng Q, He X, Lin Y. High-density genetic map construction and gene mapping of pericarp color in wax gourd using specific-locus amplified fragment (SLAF) sequencing. BMC Gen. 2015;16:1035.
5. Chang Z, Guo Y, Wang A. Study on the methods of improving the germination rate of wax gourd seeds. South China Agric. 2021;15:11–13.