Author:
Foy Niall,Jester Brian,Conant Gavin C,Devine Kevin M
Abstract
Abstract
Background
Lysyl-tRNA synthetase (LysRS) is unique within the aminoacyl-tRNA synthetase family in that both class I (LysRS1) and class II (LysRS2) enzymes exist. LysRS1 enzymes are found in Archaebacteria and some eubacteria while all other organisms have LysRS2 enzymes. All sequenced strains of Bacillus cereus (except AH820) and Bacillus thuringiensis however encode both a class I and a class II LysRS. The lysK gene (encoding LysRS1) of B. cereus strain 14579 has an associated T box element, the first reported instance of potential T box control of LysRS expression.
Results
A global study of 891 completely sequenced bacterial genomes identified T box elements associated with control of LysRS expression in only four bacterial species: B. cereus, B. thuringiensis, Symbiobacterium thermophilum and Clostridium beijerinckii. Here we investigate the T box element found in the regulatory region of the lysK gene in B. cereus strain 14579. We show that this T box element is functional, responding in a canonical manner to an increased level of uncharged tRNALys but, unusually, also responding to an increased level of uncharged tRNAAsn. We also show that B. subtilis strains with T box regulated expression of the endogenous lysS or the heterologous lysK genes are viable.
Conclusions
The T box element controlling lysK (encoding LysRS1) expression in B. cereus strain 14579 is functional, but unusually responds to depletion of charged tRNALys and tRNAAsn. This may have the advantage of making LysRS1 expression responsive to a wider range of nutritional stresses. The viability of B. subtilis strains with a single LysRS1 or LysRS2, whose expression is controlled by this T box element, makes the rarity of the occurrence of such control of LysRS expression puzzling.
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Reference35 articles.
1. Grunberg-Manago M: Regulation of the expression of aminoacyl-tRNA synthetases and translation factors. Escherichia coli and Salmonella. Cellular and Molecular Biology. Edited by: Neidhardt FC. 1996, Washington DC: ASM Press, 1432-1457.
2. Woese CR, Olsen GJ, Ibba M, Söll D: Aminoacyl-tRNA synthetases, the genetic code, and the evolutionary process. Microbiol Mol Biol Rev. 2000, 64: 202-236. 10.1128/MMBR.64.1.202-236.2000.
3. Ibba M, Söll D: The renaissance of aminoacyl-tRNA synthesis. EMBO Rep. 2000, 2: 382-387.
4. O'Donoghue P, Luthey-Schulten Z: On the evolution of structure in aminoacyl-tRNA synthetases. Microbiol Mol Biol Rev. 2003, 67: 550-573. 10.1128/MMBR.67.4.550-573.2003.
5. Ibba M, Morgan S, Curnow AW, Pridmore DR, Vothknecht UC, Gardner W, Lin W, Woese CR, Söll D: A euryarchaeal lysyl-tRNA synthetase: resemblance to class I synthetases. Science. 1997, 278: 1119-1122. 10.1126/science.278.5340.1119.
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