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Optimus Primer: A PCR enrichment primer design program for next-generation sequencing of human exonic regions

  • Published:2010-07-07 Issue:1 Volume:3 Page:
  • ISSN:1756-0500
  • Container-title:BMC Research Notes
  • language:en
  • Short-container-title:BMC Res Notes

Author:

Brown Andrew MK,Lo Ken Sin,Guelpa Paul,Beaudoin Mélissa,Rioux John D,Tardif Jean-Claude,Phillips Michael S,Lettre Guillaume

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference10 articles.

1. Ansorge WJ: Next-generation DNA sequencing techniques. N Biotechnol. 2009, 25: 195-203. 10.1016/j.nbt.2008.12.009.

2. Summerer D: Enabling technologies of genomic-scale sequence enrichment for targeted high-throughput sequencing. Genomics. 2009

3. Tsai MF, Lin YJ, Cheng YC, Lee KH, Huang CC, Chen YT, Yao A: PrimerZ: streamlined primer design for promoters, exons and human SNPs. Nucleic Acids Res. 2007, 35: W63-65. 10.1093/nar/gkm383.

4. ExonPrimer. [ http://ihg2.helmholtz-muenchen.de/ihg/ExonPrimer.html ]

5. EasyExonPrimer. [ http://129.43.22.27/~primer/EasyExonPrimer.html ]

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