Evaluation of bacteriological profile of meat contact surfaces, handling practices of raw meat and its associated factors in butcher shops of Arba Minch town, southern Ethiopia-A facility based cross sectional study

Abstract

Abstract Background Meat is subjected to contamination from a range of sources throughout animal slaughter and its sale. The demand for meat products in Ethiopia has been increased dramatically; especially the consumption of fresh chilled meat becomes a status of symbol. Objectives This study aimed to evaluate the bacteriological profile of meat contact surfaces, raw meat handling practices, and its associated factors in butcher shops located in Arba Minch town, southern Ethiopia. Methods and Materials A facility-based cross-sectional study was carried out among meat handlers in butcher shops in Arba Minch town from November to December 2020. A pre-tested and semi-structured interviewer-administered questionnaire was used to collect the data on workers meat handling practices and the sterile swab was used to collect samples from selected meat contact surfaces for bacteriological analysis. Statistical Package for Social Sciences (SPSS) version 21 was used for data analysis. Binary logistic regression analysis was utilized to assess the presence and degree of association between raw meat handling practice and independent variables. Adjusted odds ratio with a 95% confidence interval at P-value < 0.05 was considered statistically significant. Result and Conclusion This study revealed the knowledge and handling practice gap among meat handlers of butcher shops. Knowledge of food handling hygiene has shown significant association with handling practice [AOR = 4.5 (1.182,17.202)]. The median total viable count of bacterial loads from the swab samples of butchers’ hands, knives, and the tables was 5.60 ± 0.80, 5.60 ± 1.00, 5.70 ± 1.00 log10 Colony-forming unit (CFU)/cm2, respectively. Escherichia coli was the predominant isolate 34(68%) followed by Staphylococcus species 28(56%) and Salmonella species 19(38%). The median total bacterial load obtained from the samples exceeds the acceptable value of less than 5.0 log10 CFU/cm2 on fresh meat. This indicates the need for improvement in the meat handling and processing chain to safeguard the public health against the risks of foodborne bacterial infections.