Author:
Eberhardt Maria,Prochowska Sylwia,Duszewska Anna M.,Van Soom Ann,Olech Wanda,Niżański Wojciech
Abstract
Abstract
Background
The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls. Semen samples containing a large amount of functionally and morphologically impaired or dead spermatozoa have lower tolerance for cryopreservation process. Such samples are prone to involve damage acrosomes, to produce and release reactive oxygen which negatively affects proper function of spermatozoas. It is a good practice to select intact and viable gametes before subjecting the sample to cryopreservation to improve the efficiency of this process. The aim of this study was to assess the ability of Percoll® density gradient centrifugation in order to improve the quality of wisent spermatozoa after cryopreservation. Spermatozoa samples were analysed with computer-assisted semen analysis system and flow cytometry.
Results
Percoll® density gradient centrifugation resulted in increased percentage of motile spermatozoa, higher proportion of spermatozoa with normal morphology and proper functionality but also in a significant reduction of the total number of gametes. Nevertheless, the concentration of frozen spermatoza was still sufficient for obtaining a few complete insemination doses suggested for cattle from each epididymis.
Conclusions
While creating a high-quality genetic reserve, for in vitro fertilisation purposes, eliminating detritus and improving the overall quality of samples is more important than total number of spermatozoa. For these reasons, the achievement of higher post thaw quality of spermatozoa justifies the purification of samples by centrifugation in a Percoll® density gradient prior to the cryopreservation process.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine
Reference45 articles.
1. Pucek Z, Belousova I, Krasińska M, Krasiński Z, Olech W. European bison. Status Survey and Conservation Action Plan. Gland, Switzerland and Cambridge, UK IUCN/SSB Bison Specialist Group IUCN; 2004. p. 1–54.
2. Slatis M. An analysis of inbreeding in the European bison. Genetics. 1960;45:275–87.
3. Olech W. Analysis of inbreeding in European bison. Acta Theriol. 1987;32:373–87.
4. Comizzoli P, Mermillod P, Mauget R. Reproductive biotechnologies for endangered mammalian species. Reprod Nutr Dev. 2000. https://doi.org/10.1051/rnd:2000113.
5. Prieto Pablos M, Sánchez-Calabuig M, Hildebrandt T, Santiago-Moreno J. Sperm cryopreservation in wild animals. Eur J Wildl Res. 2014. https://doi.org/10.1007/s10344-014-0858-4.
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献