Author:
Ayoub Hala F.,khafagy Ahmed R.,Esawy Aboelkair M.,El-moaty Noura Abo,Alwutayd Khairiah Mubarak,Mansour Abdallah Tageldein,Ibrahim Reham A.,Abdel-moneam Dalia A.,El-Tarabili Reham M.
Abstract
AbstractIn the present study, Aeromonas hydrophila was isolated from Tilapia zillii and Mugil cephalus samples collected during different seasons from various Suez Canal areas in Egypt. The prevalence of A. hydrophila, virulence genes, and antibiotic resistance profile of the isolates to the commonly used antibiotics in aquaculture were investigated to identify multiple drug resistance (MDR) and extensive drug-resistant (XDR) strains. In addition, a pathogenicity test was conducted using A. hydrophila, which was isolated and selected based on the prevalence of virulence and resistance genes, and morbidity of natural infected fish. The results revealed that A. hydrophila was isolated from 38 of the 120 collected fish samples (31.6%) and confirmed phenotypically and biochemically. Several virulence genes were detected in retrieved A. hydrophila isolates, including aerolysin aerA (57.9%), ser (28.9%), alt (26.3%), ast (13.1%), act (7.9%), hlyA (7.9%), and nuc (18.4%). Detection of antibiotic-resistant genes revealed that all isolates were positive for blapse1 (100%), blaSHV (42.1%), tetA (60.5%), and sul1 (42.1%). 63.1% of recovered isolates were considered MDR, while 28.9% of recovered isolates were considered XDR. Some isolates harbor both virulence and MDR genes; the highest percentage carried 11, followed by isolates harboring 9 virulence and resistance genes. It could be concluded that the high prevalence of A. hydrophila in aquaculture species and their diverse antibiotic resistance and virulence genes suggest the high risk of Aeromonas infection and could have important implications for aquaculture and public health.
Publisher
Springer Science and Business Media LLC
Reference71 articles.
1. Al-Mokaddem AK, Abdel-moneam DA, Ibrahim RA, Saleh M, Shaalan M. Molecular identification, histopathological analysis and immunohistochemical characterization of non-pigmented Aeromonas salmonicida subsp. Salmonicida in Mugil Carinatus (Valenciennes, 1836). Aquacult Rep. 2022;24:101103.
2. Sreedharan K, Philip R, Singh ISB. Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health. Braz J Microbiol. 2012;43:754–65.
3. Dias MK, Sampaio LS, Proietti-Junior AA, Yoshioka ET, Rodrigues DP, Rodriguez AF, et al. Lethal dose and clinical signs of Aeromonas hydrophila in Arapaima gigas (Arapaimidae), the giant fish from Amazon. Vet Microbiol. 2016;188:12–5.
4. Hu M, Wang N, Pan Z, Lu C, Liu Y. Identity and virulence properties of Aeromonas isolates from diseased fish, healthy controls and water environment in China. Lett Appl Microbiol. 2012;55(3):224–33.
5. Zhang D, Moreira GS, Shoemaker C, Newton JC, Xu D-H. Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge. FEMS Microbiol Lett. 2016;363(9):1–5.