Author:
Lv Huifang,Peng Zhifeng,Jia Bingxin,Jing Huiyuan,Cao Sufang,Xu Zhikun,Dong Wang
Abstract
Abstract
Background
Classical swine fever (CSF) is a severe disease of pigs that results in huge economic losses worldwide and is caused by classical swine fever virus (CSFV). CSFV nonstructural protein 4 A (NS4A) plays a crucial role in infectious CSFV particle formation. However, the function of NS4A during CSFV infection is not well understood.
Results
In this study, we used RNA-seq to investigate the functional role of CSFV NS4A in PK-15 cells. A total of 3893 differentially expressed genes (DEGs) were identified in PK-15 cells expressing NS4A compared to cells expressing the empty vector (NC). Twelve DEGs were selected and further verified by RT‒qPCR. GO and KEGG enrichment analyses revealed that these DEGs were associated with multiple biological functions, including cell adhesion, apoptosis, host defence response, the inflammatory response, the immune response, and autophagy. Interestingly, some genes associated with host immune defence and inflammatory response were downregulated, and some genes associated with host apoptosis and autophagy were upregulated.
Conclusion
CSFV NS4A inhibits the innate immune response, and suppresses the expression of important genes associated with defence response to viruses and inflammatory response, and regulates cell adhesion, apoptosis and autophagy.
Funder
National Natural Science Foundation of China
Doctoral Science Foundation of Henan University of Animal Husbandry and Economy
Key Disciplines of Veterinary Medicine of Henan University of Animal Husbandry and Economy
Innovation Team of Henan University of Animal Husbandry and Economy
Key Scientific Research Project of University in Henan Province
Key Technologies Research and Development Program of Henan Province
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine