Author:
Kong Ling,Maya Walter Cardona,Moreno-Fernandez Maria E,Ma Gang,Shata Mohamed T,Sherman Kenneth E,Chougnet Claire,Blackard Jason T
Abstract
Abstract
Background
There are only limited data on whether HIV infection occurs within the liver; therefore, we explored early and late stages of the HIV life cycle in two hepatocyte cell lines – Huh7.5 and Huh7.5JFH1 – as well as in primary human hepatocytes.
Results
Integrated HIV DNA was detected in Huh7.5 and Huh7.5JFH1 cells, as well as in primary hepatocytes, and was inhibited by the integrase inhibitor raltegravir in a dose-dependent manner. HIV p24 protein was also detected in cell culture supernatants at days 1, 3, 5, and 7 post-infection and was inhibited by AZT, although levels were modest compared to those in a lymphocyte cell line. Culture supernatants from HIV-infected hepatocytes were capable of infecting a non-hepatic HIV indicator cell line.
Conclusions
These results indicating low-level HIV replication in hepatoctyes in vitro complement evidence suggesting that HIV has deleterious effects on the liver in vivo.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Cited by
46 articles.
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