Author:
Kamijo Shintaro,Hamatani Toshio,Sasaki Hiroyuki,Suzuki Hiroki,Abe Akane,Inoue Osamu,Iwai Maki,Ogawa Seiji,Odawara Kei,Tanaka Kanako,Mikashima Mutsumi,Suzuki Masami,Miyado Kenji,Matoba Ryo,Odawara Yasushi,Tanaka Mamoru
Abstract
Abstract
Objective
To generate an effective embryo prediction model and identify a non-invasive evaluation method by analyzing microRNAs (miRNAs) in embryo culture medium.
Design
Analysis of microRNA profiles from spent culture medium of blastocysts with good morphology that did or did not result in pregnancy.
Setting
Clinical and experimental research.
Patients
Sixty patients who underwent thawed embryo transfer of blastocysts after intracytoplasmic sperm injection.
Intervention(s)
None.
Main outcome measure(s)
The association of miRNA abundance levels secreted by blastocysts in culture medium and implantation success.
Results
Our RNA sequencing analysis found a total of 53 differentially expressed miRNAs in the culture media of pregnancy and non-pregnancy groups. Twenty-one miRNAs were analyzed for their potential to predict implantation success. Eight miRNAs (hsa-miR-191-5p, hsa-miR-320a, hsa-miR-92a-3p, hsa-miR-509-3p, hsa-miR-378a-3p, hsa-miR-28-3p, hsa-miR-512-5p, and hsa-miR-181a-5p) were further extracted from the results of a logistic regression analysis of qPCR Ct values. A prediction model for high-quality blastocysts was generated using the eight miRNAs, with an average accuracy of 0.82 by 5-fold cross validation.
Conclusion
We isolated blastocyst miRNAs that may predict implantation success and created a model to predict viable embryos. Increasing the number of investigated cases and further studying the effect of each miRNA on embryonic development is needed to refine the miRNA-based predictive model.
Publisher
Springer Science and Business Media LLC
Subject
Developmental Biology,Endocrinology,Reproductive Medicine,Obstetrics and Gynecology
Reference42 articles.
1. Machtinger R, Laurent LC, Baccarelli AA. Extracellular vesicles: roles in gamete maturation, fertilization and embryo implantation. Hum Reprod Update. 2016;22:182–93.
2. Harada Y, Yoshida K, Kawano N, Miyado K. Critical role of exosomes in sperm-egg fusion and virus-induced cell-cell fusion. Reprod Med Biol. 2013;12:117–26.
3. Kawano N, Miyado K, Yoshii N, Kanai S, Saito H, Miyado M, et al. Absence of CD9 reduces endometrial VEGF secretion and impairs uterine repair after parturition. Sci Rep. 2014;4:4701.
4. Quenby S, Brosens JJ. Human implantation: a tale of mutual maternal and fetal attraction. Biol Reprod. 2013;88:81.
5. Haouzi D, Mahmoud K, Fourar M, Bendhaou K, Dechaud H, De Vos J, et al. Identification of new biomarkers of human endometrial receptivity in the natural cycle. Hum Reprod. 2009;24:198–205.
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