Author:
Shu Chongyi,Yu Yiqi,Xiaopan Chen ,Guo Jiansheng,Zhou Yier,Wu Dandan,Yang Tianyun,Fan Yuhang,Huang Qiongxiao,Shu Jing
Abstract
AbstractThe oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In this study, we studied the internal structures and cell-to-cell connections of mouse cGCs using focused ion beam scanning electron microscopy (FIB-SEM). We reconstructed three-dimensional models to display characteristic connections between the oocyte and cGCs, and to illustrate various main organelles in cGCs together with their interaction relationship. A special form of cilium identified in granulosa cell was never reported in previous literature.
Funder
the Medicine and Health Research Foundation of Zhejiang Province
Publisher
Springer Science and Business Media LLC
Subject
Obstetrics and Gynecology,Oncology