The immune status of rabbits primed with chicken thymic protein

Abstract

Chicken thymus glands were collected from three weeks old chicken through standard manual dissection technique.T he glands were minced, mortared, centrifuged at 5000 RPM for 15 min .and supernatant was saved.T hymic protein was separated by PEG 6%( M.w 6000) solution.T his protein is designed as chicken thymic protein (CTP). CTP was used a test immunogen and sensitizer.A s an immunogen, it was used in a concentration, 1.1g/land 0.55 g/l for priming of rabbits using multi-site injection protocol with Freund's complete adjuvant.Passive haemagglutination, NBT , LIF ,E- rosette and skin test were made on CTP primed rabbits and controls. It was found that CTP could act as a humoral immunogen, T cell suppressor and LIF cytokine inducer. Thus CTP may contain lapin immunoregulatory protein