Protective Effect of Caffeic Acid Phenethyl Ester on Antituberculosis Drug-Induced Hepatotoxicity in Rats

Abstract

Isoniazid and rifampicin are drugs primarily used in antituberculosis treatment. Our aim in this study is to evaluate the effect of caffeic acid phenethyl ester's protective effect on liver function tests and to trace elements in hepatic damage caused by isoniazid and rifampicin on rats. Forty Wistar albino rats were divided into 4 groups. Group 1: Sham, Group 2: caffeic acid phenethyl ester application, Group 3: isoniazid and rifampicin given, Group 4: isoniazid + rifampicin and caffeic acid phenethyl ester application. After 30 days, the rats were sacrificed by taking blood from the heart. Alanine aminotransferase, aspartate aminotransferase, zinc, copper, total antioxidant capacity, total oxidative status, and oxidative stress index levels were evaluated. The rats to which isoniazid + rifampicin+ caffeic acid phenethyl ester were given had less oxidative stress and copper levels (P < 0.001, P = 0.019) but have higher zinc levels (P = 0.001) compared to the isoniazid + rifampicin group. Liver enzyme levels were also lower in rats that were given isoniazid + rifampicin + caffeic acid phenethyl ester (P < 0.001). The results of this study suggested that caffeic acid phenethyl ester influences the levels of trace elements (copper and zinc) that are important for the physiologic mechanisms of organisms, reducing liver damage.