The Extraction of Antioxidant Compounds from Coriandrum sativum Seeds by Using Green Solvents

Abstract

In this study, bioactive compounds from Coriandrum sativum seeds were extracted by microwave assisted extraction (MAE) using natural deep eutectic solvents (NADESs). The total antioxidant capacity (TAC) of extracts was determined by using cupric reducing antioxidant capacity (CUPRAC) method. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) analyses have been employed to measure the free radical scavenging ability of the sample extracts. Five different deep eutectic solvents, using choline chloride in combination with hydrogen bond donors (three polyalcohols and two organic acids) were firstly scanned. Choline chloride and 1,4-butanediol at the molar ratio of 1:4 was the best solvent of choice to extract natural antioxidants to achieve the best level of TAC. The response surface methodology (RSM) was applied to achieve the most advantageous conditions. The optimal process conditions for the maximum TAC value were as follows: 326 watt microwave power, 88 second extraction time, and 10 liquid/solid (L/S) ratio. In this study, we report an efficient, rapid, and green method to extract natural antioxidants from Turkish Coriandrum sativum seeds.