Affiliation:
1. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, China
2. Luzhou Modern Agriculture Development Promotion Center, Luzhou, China
3. Chengdu Biotechservice Institute, Chengdu, China
4. Sichuan Dekon Livestock Foodstuff Group, Zigong, China
5. Luzhou Agricultural and Rural Bureau, Luzhou, China
6. Tongjiang County Animal Husbandry Station, Bazhong, China
Abstract
Neijiang (NJ) and Yacha (YC) are two indigenous pig breeds in the Sichuan basin of China, displaying higher resistance to diseases, lower lean ratio, and slower growth rate than the commercial Western pig breed Yorkshire (YS). The molecular mechanisms underlying the differences in growth and development between these pig breeds are still unknown. In the present study, five pigs from NJ, YC, and YS breeds were subjected to the whole genome resequencing, and then the differential single-nucleotide polymorphisms (SNPs) were screened using a 10-kb window sliding in 1-kb step using the Fst method. Finally, 48,924, 48,543, and 46,228 nonsynonymous single-nucleotide polymorphism loci (nsSNPs) were identified between NJ and YS, NJ and YC, and YC and YS, which highly or moderately affected 2,490, 800, and 444 genes, respectively. Moreover, three nsSNPs were detected in the genes of acetyl-CoA acetyltransferase 1 ( ACAT1) insulin-like growth factor 2 receptor ( IGF2R), insulin-like growth factor 2 and mRNA-binding protein 3 ( IGF2BP3), which potentially affected the transformation of acetyl-CoA to acetoacetyl-CoA and the normal functions of the insulin signaling pathways. Moreover, serous determinations revealed significantly lower acetyl-CoA content in YC than in YS, supporting that ACAT1 might be a reason explaining the differences in growth and development between YC and YS breeds. Contents of phosphatidylcholine (PC) and phosphatidic acid (PA) significantly differed between the pig breeds, suggesting that glycerophospholipid metabolism might be another reason for the differences between Chinese and Western pig breeds. Overall, these results might contribute basic information to understand the genetic differences determining the phenotypical traits in pigs.
Funder
Sichuan Science and Technology Innovation Talents
Science & Technology Support Program of Sichuan
Program for Pig Industry Technology System Innovation Team of Sichuan Province
Science and Technology Project of Animal Science Academy
Special Finance of Sichuan
China Agriculture Research System
National Key Research and Development Program of China
Science and Technology Major Projects of Sichuan
National Natural Science Foundation of China
Publisher
American Physiological Society
Cited by
3 articles.
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