Proteomics of novel induced pluripotent stem cell-derived vascular endothelial cells reveal extensive similarity with an immortalized human endothelial cell line-Reference-Cited by-同舟云学术

Proteomics of novel induced pluripotent stem cell-derived vascular endothelial cells reveal extensive similarity with an immortalized human endothelial cell line

Published:2023-08-01 Issue:8 Volume:55 Page:324-337
ISSN:1094-8341
Container-title:Physiological Genomics
language:en
Short-container-title:Physiological Genomics

Author:

Ariyasinghe Nethika R.¹²,de Souza Santos Roberta³⁴,Gross Andrew³⁴,Aghamaleky-Sarvestany Arwin³⁴,Kreimer Simion¹²,Escopete Sean¹,Parker Sarah J.¹²⁵^ORCID,Sareen Dhruv³⁴⁶²⁵^ORCID

Affiliation:

1. Department of Cardiology, Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, United States

2. Board of Governors Innovation Center, Cedars-Sinai Medical Center, Los Angeles, California, United States

3. Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, United States

4. Cedars-Sinai Biomanufacturing Center, Cedars-Sinai Medical Center, Los Angeles, California, United States

5. Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, California, United States

6. iPSC Core, Cedars-Sinai Medical Center, Los Angeles, California, United States

Abstract

We have developed methods to differentiate induced pluripotent stem cells (iPSCs) across multiple iPSC lines into nontissue-specific pan vascular ECs (iECs) and demonstrated the proteomic similarity of these cells to a widely used endothelial cell line (HUVECs). We also identified posttranslational modifications and targets for increasing the proteomic similarity of iECs to HUVECs. In the future, iECs can be used to study EC development, signaling, and metabolism for future regenerative applications.

Funder

Cedars-Sinai Programmatic Funds

California Institute for Regenerative Medicine

Publisher

American Physiological Society

Subject

Genetics,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/physiolgenomics.00166.2022

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