Centrally administered neuropeptide Y delays gastric emptying via Y2receptors in rats

Abstract

It has been shown that centrally administered neuropeptide Y (NPY) delays gastric emptying. To determine the receptor subtypes of NPY mediating the inhibitory effects on gastric emptying, effects of intracerebroventricular injection of NPY, [Leu31,Pro34]NPY (a Y1agonist) and NPY-(3–36) (a Y2agonist) on solid gastric emptying and postprandial antropyloric motility were studied in conscious rats. Intracerebroventricular injection of NPY and NPY-(3–36), but not [Leu31,Pro34] NPY, delayed solid gastric emptying in a dose-dependent manner (0.03–3 nmol). After the feeding (40 min), contractions with low frequency and high amplitude of the antrum were frequently observed, and the peak contraction of the antrum occurred most often 3–6 s before the peak contraction of the pylorus. Intracerebroventricular injection of NPY and NPY-(3–36) (3 nmol), but not [Leu31,Pro34]NPY, significantly reduced antral contractions and the number of antropyloric coordination events. It is suggested that centrally administered NPY impairs postprandial antral contractions and antropyloric coordination via Y2receptors, resulting in delayed gastric emptying.