Anesthetics can alter subsequent in vitro assessment of contractility in slow and fast skeletal muscles of rat

Author:

Lapointe Benoît M.1,Côté Claude H.1

Affiliation:

1. Laval University Hospital Research Center, Ste-Foy, Québec, Canada G1V 4G2

Abstract

Anesthetic agents can interfere with measurement of skeletal muscle contractility in vivo or in situ. Data obtained in vitro are however believed to be unaffected by such drugs. Our objective was to compare in vitro contractile measurements of fast- and slow-twitch muscles dissected from rats anesthetized with pentobarbital sodium (PS, 50 mg/kg ip) or with a mixture of ketamine and xylazine (KX, 87.5:12.5 mg/kg ip). The soleus (Sol) and extensor digitorum longus (EDL) muscles were precisely dissected 10 and 20 min after induction of anesthesia and equilibrated for 20 min in vitro before measuring contractile properties. All data obtained from PS rats were comparable with published values obtained under similar conditions. In EDL, maximum tetanic tension (Po) in KX rats was significantly decreased at both times compared with that in PS muscles. In the Sol, only the muscles exposed for 20 min to KX showed a decreased Po. These results clearly emphasize the need for investigators assessing skeletal muscle contractility in vitro to take into account the type of anesthetics used and the time of in vivo exposition to the drug.

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3