Effects of CO-2 and extracellular H+ iontophoresis on single cell activity in the cat brainstem

Abstract

The activity of single cells in deep regions of the medulla oblongata was observed both during CO2 inhalation and during the extracellular iontophoresis of hydrogen ions in peripheral chemoreceptor-denervated cats. All 53 neurons that fired in synchrony with some part of the ventilatory cycle showed increased firing during CO2 inhalation; yet none responded in a graded fashion to the extracellular application of hydrogen ions. Seventy-one of the 74 nonperiodic cells studied showed no response to CO2 inhalation. Of the 3 nonperiodic cells that did respond to CO2, 2 also responded in a graded fashion to the extracellular iontophoresis of hydrogen ions. It is concluded that the cell bodies of medullary neurons with respiratory periodicity are relatively insensitive to hydrogen ions. Further the paucity of hydrogen ion-sensitive cells found in deep areas of the medulla does not support the notion that medullary hydrogen ion chemoreception is largely achieved by structures located deep in the lower brainstem.