A biologically validated mathematical model for decoding epithelial apical, basolateral, and paracellular electrical properties

Abstract

This interdisciplinary paper integrates mathematics, biology, and physiology to measure epithelial tissue’s apical, basolateral, and paracellular transport pathways. A key advancement is the inclusion of intracellular voltage recordings using a sharp pipette, enabling precise quantification of relative impedance changes between apical and basolateral membranes. This enhanced electrochemical impedance spectroscopy technique offers insights into epithelial transport dynamics, advancing disease understanding, drug interactions, and cell therapies. Its broad applicability contributes significantly to epithelial physiology research.