Altered pHiregulation and Na+/HCO3−transporter activity in choroid plexus of cilia-defectiveTg737orpkmutant mouse

Abstract

Tg737orpkmice have defects in cilia assembly and develop hydrocephalus in the perinatal period of life. Hydrocephalus is progressive and is thought to be initiated by abnormal ion and water transport across the choroid plexus epithelium. The pathology is further aggravated by the slow and disorganized beating of motile cilia on ependymal cells that contribute to decreased cerebrospinal fluid movement through the ventricles. Previously, we demonstrated that the hydrocephalus phenotype is associated with a marked increase in intracellular cAMP levels in choroid plexus epithelium, which is known to have regulatory effects on ion and fluid movement in many secretory epithelia. To evaluate whether the hydrocephalus in Tg737orpkmutants is associated with defects in ion transport, we compared the steady-state pHiand Na+-dependent transport activities of isolated choroid plexus epithelium tissue from Tg737orpkmutant and wild-type mice. The data indicate that Tg737orpkmutant choroid plexus epithelium have lower pHiand higher Na+-dependent HCO3−transport activity compared with wild-type choroid plexus epithelium. In addition, wild-type choroid plexus epithelium could be converted to a mutant phenotype with regard to the activity of Na+-dependent HCO3−transport by addition of dibutyryl-cAMP and mutant choroid plexus epithelium toward the wild-type phenotype by inhibiting PKA activity with H-89. Together, these data suggest that cilia have an important role in regulating normal physiology of choroid plexus epithelium and that ciliary dysfunction in Tg737orpkmutants disrupts a signaling pathway leading to elevated intracellular cAMP levels and aberrant regulation of pHiand ion transport activity.