Phosphorylation of AS160-serine 704 is not essential for exercise-increase in insulin-stimulated glucose uptake by skeletal muscles from female or male rats-Reference-Cited by-同舟云学术

Phosphorylation of AS160-serine 704 is not essential for exercise-increase in insulin-stimulated glucose uptake by skeletal muscles from female or male rats

Published:2024-06-01 Issue:6 Volume:326 Page:E807-E818
ISSN:0193-1849
Container-title:American Journal of Physiology-Endocrinology and Metabolism
language:en
Short-container-title:American Journal of Physiology-Endocrinology and Metabolism

Author:

Wang Haiyan¹^ORCID,Kwak Seong Eun¹^ORCID,Zheng Amy¹,Arias Edward B.¹,Pan Xiufang²,Duan Dongsheng²³⁴⁵,Cartee Gregory D.¹⁶⁷^ORCID

Affiliation:

1. Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, Michigan, United States

2. Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, Missouri, United States

3. Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States

4. Department of Neurology, School of Medicine, University of Missouri, Columbia, Missouri, United States

5. Department of Chemical and Biomedical Engineering, College of Engineering, University of Missouri, Columbia, Missouri, United States

6. Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, United States

7. Institute of Gerontology, University of Michigan, Ann Arbor, Michigan, United States

Abstract

The current study evaluated the role of Akt substrate of 160 kDa (AS160) phosphorylation on Ser704 in increased insulin-stimulated glucose uptake by skeletal muscle after exercise. Adeno-associated virus vectors were engineered to express either wild-type-AS160 or AS160 mutated so that it could not be phosphorylated on Ser704 in paired muscles from AS160-knockout rats. The results demonstrated that AS160 phosphorylation on Ser704 was not essential for exercise-induced elevation in insulin-stimulated glucose uptake by rats of either sex.

Funder

HHS | National Institutes of Health

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpendo.00010.2024

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