Repeated Cocaine Administration Suppresses HVA-Ca2+Potentials and Enhances Activity of K+Channels in Rat Nucleus Accumbens Neurons

Abstract

The nucleus accumbens (NAc) is an important forebrain area involved in sensitization, withdrawal effects, and self-administration of cocaine. However, little is known about cocaine-induced alterations in the neuronal excitability and whole cell neuroplasticity in this region that may affect behaviors. Our recent investigations have demonstrated that repeated cocaine administration decreases voltage-sensitive sodium and calcium currents (VSSCs and VSCCs, respectively) in freshly dissociated NAc neurons of rats. In this study, current-clamp recordings were performed in slice preparations to determine the effects of chronic cocaine on evoked Ca2+potentials and voltage-sensitive K+currents in NAc neurons. Repeated cocaine administration with 3–4 days of withdrawal caused significant alterations in Ca2+potentials, including suppression of Ca2+-mediated spikes, increase in the intracellular injected current intensity required for generation of Ca2+potentials (rheobase), reduced duration of Ca2+plateau potentials, and abolishment of secondary Ca2+potentials associated with the primary Ca2+plateau potential. Application of nickel (Ni2+), which blocks low-voltage activated T-type Ca2+channels, had no impact on evoked Ca2+plateau potentials in NAc neurons, indicating that these Ca2+potentials are high-voltage activated (HVA). In addition, repeated cocaine pretreatment also hyperpolarized the resting membrane potential, increased the amplitude of afterhyperpolarization in Ca2+spikes, and enhanced the outward rectification observed during membrane depolarization. These findings indicate that repeated cocaine administration not only suppressed HVA-Ca2+potentials but also significantly enhanced the activity of various K+channels in NAc neurons. They also demonstrate an integrative role of whole cell neuroplasticity during cocaine withdrawal, by which the subthreshold membrane excitability of NAc neurons is significantly decreased.