Homeostatic responses to caloric restriction: influence of background metabolic rate

Abstract

The biological responses to caloric restriction (CR) are generally examined in rats with elevated metabolic rates due to being housed at ambient temperatures (Ta) below the zone of thermoneutrality. We determined the physiological and behavioral responses to 2 wk of 30–40% CR in male FBNF1 rats housed in cool (Ta = 12°C) or thermoneutral (TMN; Ta = 30°C) conditions. Rats were instrumented with telemetry devices and housed continuously in home-cage calorimeters for the entire experiment. At baseline, rats housed in cool Ta had reduced rate of weight gain; thus a mild CR (5%) group at thermoneutrality for weight maintenance was also studied. Rats housed in cool Ta exhibited elevated caloric intake (cool = 77 ± 1; TMN = 54 ± 2 kcal), oxygen consumption (V̇o2; cool = 9.9 ± 0.1; TMN = 5.5 ± 0.1 ml/min), mean arterial pressure (cool = 103 ± 1; TMN = 80 ± 2 mmHg), and heart rate (cool = 374 ± 3; TMN = 275 ± 4 beats/min). Cool-CR rats exhibited greater CR-induced weight loss (cool = −62 ± 3; TMN = −42 ± 3 g) and reductions in V̇o2 (cool = −2.6 ± 0.1; TMN = −1.5 ± 0.1 ml/min) but similar CR-induced reductions in heart rate (cool = −59 ± 1; TMN= −51 ± 7 beats/min). CR had no effect on arterial blood pressure or locomotor activity in either group. Unexpectedly, weight maintenance produced significant reductions in V̇o2 and heart rate. At thermoneutrality, a single day of refeeding effectively abolished CR-induced reductions in V̇o2 and heart rate. The results reveal that rats with low or high baseline metabolic rate exhibit comparable compensatory reductions in V̇o2 and heart rate and suggest that Ta can be used to modulate the metabolic background on which the more prolonged effects of CR can be studied.