hiPSC-CM electrophysiology: impact of temporal changes and study parameters on experimental reproducibility-Reference-Cited by-同舟云学术

hiPSC-CM electrophysiology: impact of temporal changes and study parameters on experimental reproducibility

Published:2024-07-01 Issue:1 Volume:327 Page:H12-H27
ISSN:0363-6135
Container-title:American Journal of Physiology-Heart and Circulatory Physiology
language:en
Short-container-title:American Journal of Physiology-Heart and Circulatory Physiology

Author:

Guerrelli Devon¹²³^ORCID,Pressman Jenna¹²^ORCID,Salameh Shatha¹³,Posnack Nikki¹³⁴^ORCID

Affiliation:

1. Sheikh Zayed Institute for Pediatric Surgical Innovation, Children’s National Hospital, Washington, District of Columbia, United States

2. Department of Biomedical Engineering, The George Washington University School of Engineering and Applied Science, Washington, District of Columbia, United States

3. Children’s National Heart Institute, Children’s National Hospital, Washington, District of Columbia, United States

4. Department of Pediatrics, Department of Pharmacology and Physiology, The George Washington University School of Medicine and Health Sciences, Washington, District of Columbia, United States

Abstract

We demonstrate that iCell cardiomyocytes2 electrophysiology measurements are impacted by deviations in experimental techniques including electrical stimulation protocols, equilibration time, well-to-well variability, and length of hiPSC-CM culture. Furthermore, our results indicate that hiPSC-CM drug responsiveness changes within the first 2 wk following defrost.

Funder

American Heart Association

HHS | National Institutes of Health

Children’s National Heart Institute

Children’s Research Institute

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpheart.00631.2023

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