Author:
Trost Nina,Stepisnik Tina,Berne Sabina,Pucer Anja,Petan Toni,Komel Radovan,Debeljak Natasa
Abstract
AbstractBackground. Functional erythropoietin (EPO) signaling is not specific only to erythroid lineages and has been confirmed in several solid tumors, including breast. Three different isoforms of erythropoietin receptor (EPOR) have been reported, the soluble (EPOR-S) and truncated (EPOR-T) forms acting antagonistically to the functional EPOR. In this study, we investigated the effect of human recombinant erythropoietin (rHuEPO) on cell proliferation, early gene response and the expression of EPOR isoforms in the MCF-7 breast cancer cell line.Materials and methods. The MCF-7 cells were cultured with or without rHuEPO for 72 h or 10 weeks and assessed for their growth characteristics, expression of early response genes and different EPOR isoforms. The expression profile of EPOR and EPOR-T was determined in a range of breast cancer cell lines and compared with their invasive properties.Results. MCF-7 cell proliferation after rHuEPO treatment was dependent on the time of treatment and the concentration used. High rHuEPO concentrations (40 U/ml) stimulated cell proliferation independently of a preceding long-term exposure of MCF-7 cells to rHuEPO, while lower concentrations increased MCF-7 proliferation only after 10 weeks of treatment. Gene expression analysis showed activation of EGR1 and FOS, confirming the functionality of EPOR. rHuEPO treatment also slightly increased the expression of the functional EPOR isoform, which, however, persisted throughout the 10 weeks of treatment. The expression levels of EPOR-T were not influenced. There were no correlations between EPOR expression and the invasiveness of MCF-7, MDA-MB-231, Hs578T, Hs578Bst, SKBR3, T-47D and MCF-10A cell lines.Conclusions. rHuEPO modulates MCF-7 cell proliferation in time- and concentration-dependent manner. We confirmed EGR1, FOS and EPOR as transcription targets of the EPO-EPOR signaling loop, but could not correlate the expression of different EPOR isoforms with the invasiveness of breast cancer cell lines.
Subject
Radiology, Nuclear Medicine and imaging,Oncology
Reference56 articles.
1. In vivo evidence that erythropoietin protects neurons from ischemic damage;Sakanaka;Proc Natl Acad Sci USA,1998
2. The guidelines minimum information for publication of quantitative realtime PCR experiments;Bustin;Clin Chem,2009
3. language and environment for statistical Fundation for Statistical;RDCT;computing Computing,2008
4. The role of erythropoietin and its receptor in growth survival and therapeutic response of human tumor cells From clinic to bench - a critical;Szenajch;review Biochim Biophys Acta,2010
5. Erythropoietin can promote erythroid progenitor survival by repressing apoptosis through XL and;Silva;Blood,1996
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