Molecular characterization of Bertiella studeri infecting a primate in South India

Author:

Sebasteena P. F.12,Deepa C. K.1,Varghese A.1,Ajith Kumar K. G.1,Joy A.12,Iype A.12,Rajappan A.3,Chandy G.2,Ravindran R.1

Affiliation:

1. Department of Veterinary Parasitology, College of Veterinary and Animal Sciences , Pookode , Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India ,

2. Centre for Wildlife studies , Kerala Veterinary and Animal Sciences University , Pookode , Wayanad , Kerala , India

3. Department of Veterinary Pathology, College of Veterinary and Animal Sciences , Pookode , Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India

Abstract

Summary Bertiella spp. is a mite-borne cestode parasite that inhabits the small intestine of wide range of mammals, including non-human primates. In the present study, the morphological and molecular analysis of Bertiella studeri recovered from the small intestine of a bonnet macaque (Macaca radiata) from Wayanad, Kerala (South India) was performed. Acetic alum carmine staining identified the cestode morphologically based on the characters like broader proglottids, which contain irregularly alternating genital pores, single set of reproductive organs, 280 testes and a tubular transverse uterus. Molecular characterization was done using 18SrRNA, ITS1-5.8S and COX1 genes. Phylogenetic trees were constructed using MEGA X based on the Maximum Likelihood (ML) method (Hasegawa-Kishino-Yano (HKY) model). Cytochrome oxidase I gene could detect the existence of genetic variation in the parasite from two different hosts viz., monkey (Kerala, Argentina, and Kenya) and human (Sri Lanka). A minimum spanning network of haplotypes was generated by the haplotype networking with the above sequences using the popARTv1.7. Haplotype analysis based on COX1 revealed that the parasite haplotype was different in each country with highest population frequency in Sri Lanka.

Publisher

Walter de Gruyter GmbH

Reference18 articles.

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