Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)
Author:
Satyaprakash K.12, Khan W. A.1, Zade N. N.1, Chaudhari S. P.1, Shinde S. V.1, Kurkure N. V.3, Shembalkar P. K.4
Affiliation:
1. Department of Veterinary Public Health & Epidemiology , Nagpur Veterinary College , Nagpur , Maharashtra , India - 2. Department of Veterinary Public Health & Epidemiology, Faculty of Veterinary and Animal Sciences , Banaras Hindu University , Barkachha , Mirzapur , Uttar Pradesh , India - 3. Department of Veterinary Pathology , Nagpur Veterinary College , Nagpur , Maharashtra , India - 4. Get Well Hospital and Research Institute , Nagpur , Maharashtra , India -
Abstract
SummaryNeurocysticercosis (NCC), one of the most important neuroparasitic diseases in humans, is caused byCysticercus cellulosae, the metacestode stage of digenetic zoonotic cestodeTaenia solium. The present study aims at the detection of anti-cysticercus antibodies in the sera of epileptic patients (n=26) visiting a tertiary care hospital in Nagpur, Maharashtra state, India, by an in-house developed indirect IgG-ELISA and enzyme-linked immunoelectro transfer blot (EITB) assay using different antigens (namely, Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA), Excretory-Secretory Antigen (ESA) and Membrane-Body Antigen (MBA)) prepared fromT. soliummetacestodes to find out the status of NCC. An attempt has also been made for molecular detection of NCC from blood samples of those patients by Polymerase Chain Reaction (PCR) assay targeted atlarge subunit rRNAgene ofT. solium. The IgG ELISA level of anti-cysticercus antibodies against WCA, CFA, SA, ESA and MBA antigens were as follows: 19.23 %, 23.07 %, 38.46 %, 30.76 % and 15.38 %. The seroreactivity to CFA, SA and ESA was found in equal proportions in patients with ring-enhancing lesions. In the EITB assay, the lower and medium molecular weight protein bands of SA and ESA were immunodominant compared to the higher WCA and CFA peptides. PCR positivity could be observed in 34.6 % (9/26) of the patients under study. It is the first report of detecting NCC among epileptic patients of the Nagpur region of Maharashtra state in India using serological and molecular tools.
Publisher
Walter de Gruyter GmbH
Subject
Animal Science and Zoology,Parasitology
Reference96 articles.
1. Almeida, C.R., Ojopi, E.P., Nunes, C.M., Machado, L.R., Takayanagui, O.M., Livramento, J.A., Abraham, R., Gattaz, W.F., Vaz, A.J., Dias-Neto, E. (2006): Taenia solium DNA is present in the cerebrospinal fluid of neurocysticercosis patients and can be used for diagnosis. Eur Arch Psychiatry Clin Neurosci, 256(5): 307 – 310. DOI: 10.1007/s00406-006-0612-3 2. Aluja, A.S., Villalobos, A.N.M., Plancarte, A., Rodarte L.F., Hernández, M., Sciutto, E. (1996): Experimental Taenia solium cysticercosis in pigs: characteristics of the infection and antibody response. Vet Parasitol, 61: 49 – 59. DOI: 10.1016/0304-4017(95)00817-9 3. Arruda, G.C., Da Silva, A.D.T., Quagliato, E.M.A.B., Maretti, M.A., Rossi, C.L. (2005) Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for the serodiagnosis of neurocysticercosis. Trop Med Int Health, 10(10): 1005 – 1012. DOI: 10.1111/j.1365-3156.2005.01480.x 4. Atluri, S.R.V., Gogulamudi, V.R., Singhi, P., Khandelwal, N., Parasa, L.S., Malla, N. (2014): Pediatric neurocysticercosis: Usefulness of antibody response in cysticidal treatment follow-up. BioMed Res Int, 2014: 904046. DOI: 10.1155/2014/904046 5. Atluri, S.R.V., Singhi, P., Khandelwal, N., Malla, N. (2009): Neurocysticercosis immunodiagnosis using Taenia solium cysticerci crude soluble extract, excretory secretory and lower molecular mass antigens in serum and urine samples of Indian children. Acta Trop, 110(1): 22 – 27. DOI: 10.1016/j.actatropica.2008.12.004
|
|