Study of catalase immobilized on a silicate matrix for non-aqueous biocatalysis-Reference-Cited by-同舟云学术

Study of catalase immobilized on a silicate matrix for non-aqueous biocatalysis

Published:2005-06-01 Issue:2 Volume:3 Page:279-287
ISSN:2391-5420
Container-title:Open Chemistry
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Author:

Horozova Elena¹,Dimcheva Nina¹

Affiliation:

1. 1Department of Physical Chemistry, Plovdiv University, 24, Tsar Assen st., 4000, Plovdiv, Bulgaria

Abstract

AbstractCatalytic activity of catalase (CAT) immobilized on a modified silicate matrix to mediate decomposition of meta-chloroperoxibenzoic acid (3-CPBA) in acetonitrile has been investigated by means of quantitative UV-spectrophotometry. Under the selected experimental conditions, the kinetic parameters: the apparent Michaelis constat (KM), the apparent maximum rate of enzymatic reaction (Vmaxapp), the first order specific rate constants (ksp), the energy of activation (Ea) and the pre-exponential factor of the Arrhenius equation (Z0) were calculated. Conclusions regarding the rate-limiting step of the overall catalytic process were drawn from the calculated values of the Gibbs energy of activation ΔG*, the enthalpy of activation ΔH*, and the entropy of activation ΔS*.

Publisher

Walter de Gruyter GmbH

Subject

Materials Chemistry,General Chemistry

Link

http://link.springer.com/content/pdf/10.2478/BF02475996.pdf

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