Lack of salidroside impact on selected cytochromes encoding genes transcription in the liver of ethanol induced rats-Reference-Cited by-同舟云学术

Lack of salidroside impact on selected cytochromes encoding genes transcription in the liver of ethanol induced rats

Published:2021-09-01 Issue:3 Volume:67 Page:53-65
ISSN:2449-8343
Container-title:Herba Polonica
language:en
Short-container-title:

Author:

Kujawski Radosław¹^ORCID,Szulc Michał¹^ORCID,Toboła Maria¹,Graczyk Marcin¹,Czora-Poczwardowska Kamila¹^ORCID,Baraniak Justyna²^ORCID,Kania-Dobrowolska Małgorzata²^ORCID,Słyńko-Krzyżostaniak Julia¹^ORCID,Krajewska-Patan Anna³^ORCID,Mikołajczak Przemysław Ł.¹²^ORCID

Affiliation:

1. Department of Pharmacology , Poznan University of Medical Sciences , Rokietnicka 5a, 60-806 Poznań , Poland

2. Department of Pharmacology and Phytochemistry , Institute of Natural Fibres and Medicinal Plants National Research Institute , Kolejowa 2, 62-064 Plewiska , Poland

3. Department of Breeding and Botany of Useful Plants , Institute of Natural Fibres and Medicinal Plants National Research Institute , Kolejowa 2, 62-064 Plewiska , Poland

Abstract

Summary Introduction: The molecular basis of in vivo metabolism of selected representatives of phenylethanoids in the presence of ethanol has not been fully elucidated. Objective: The aim was to estimate a salidroside (Sal) metabolism in the liver tissue in rats with induced alcohol tolerance by assessing changes in the transcription of genes encoding cytochromes: CYP1A2, 2D2, 3A1, 2C23. Methods: cDNA was synthesized from total RNA isolated from rat liver samples. mRNA level changes were evaluated using real-time PCR (qRT-PCR) technique. Results: Ethanol caused a significant induction of the CYP1A2 and CYP2C23 genes transcription, and a decrease in the CYP3A1 mRNA level, predominantly without statistical significance. A statistically significant increase of the CYP1A2 mRNA level was observed in the group receiving only Sal (4.5 mg/kg b.w.; p.o.) (p<0.01). Conclusions: There was no unequivocal effect of salidroside on the transcription of investigated cytochrome genes in the liver of rats with induced alcohol tolerance.

Publisher

Walter de Gruyter GmbH

Link

https://www.sciendo.com/pdf/10.2478/hepo-2021-0016

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