Assessment of Antimicrobial Resistance of Salmonella spp. Isolated from Farmed Asian Clam Corbicula Fluminea

Abstract

Abstract In the present study, Salmonella spp. was successfully isolated from Asian clam Corbicula fluminea by using Xylose Lysine Deoxycholate (XLD) selective agar. A total of 200 bacterial colonies from live Asian clams were isolated and subjected to antimicrobial sensitivity test by using disc diffusion method. A total of 18 antibiotics was applied in the present study, namely oxolinic acid, nalidixic acid, erythromycin, tetracycline, doxycycline, oleandomycin, oxytetracycline, spiramycin, ampicillin, kanamycin, fosfomycin, florfenicol, lincomycin, novobiocin, chloramphenicol, amoxycillin, flumequine and sulphamethoxazole. The findings of the present study showed that total antibiotic sensitive case for Salmonella spp. isolated from C. fluminea sample was 50% or 1800 cases. This was followed by antibiotic resistance case 45% or 1620 cases and intermediary antibiotic sensitive case (5% or 180 cases). Based on the results of the present study, tetracycline, doxycycline, oxytetracycline and flumequine showed the highest inhibition of isolated Salmonella spp. The multiple antibiotic resistance (MAR) index was 0.36, indicating the sampled clams were highly exposed to the tested antibiotics.