Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2-Reference-Cited by-同舟云学术

Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2

Published:2022-06-18 Issue:s1 Volume:69 Page:87-89
ISSN:2453-6725
Container-title:European Pharmaceutical Journal
language:en
Short-container-title:

Author:

Huniadi Mykhailo¹,Salanci Simon¹,Sykora Jan²,Kurhajec Slavomir³,Sarissky Marek¹

Affiliation:

1. Department of Pharmacology, Faculty of Medicine , P.J. Safarik University , Kosice , Slovak Republic

2. Department of Hematology and Oncohematology, L. Pasteur University Hospital and Faculty of Medicine , P.J. Safarik University , Kosice , Slovak Republic

3. Department of Pharmaceutical Technology, Pharmacognosy and Pharmaceutical Botany , University of Veterinary Medicine and Pharmacy , Kosice , Slovak Republic

Abstract

Abstract SAP and EAT-2 are intracellular adaptor proteins that link SLAMF receptors to downstream intracellular signalling pathways. Their expression and functions vary among different types of hematopoietic cells. The roles of SAP and EAT-2 in haematological malignancies are poorly understood. Here, we attempted to optimise a method for the detection of intracellular SAP and EAT-2 by flow cytometry. We found that using PerFix EXPOSE kit for cell fixation and permeabilization ensures optimal results in terms of staining intensity and signal-to-noise ratio.

Publisher

Walter de Gruyter GmbH

Subject

General Pharmacology, Toxicology and Pharmaceutics

Link

https://www.sciendo.com/pdf/10.2478/afpuc-2022-0007

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