Mycobacteriosis in Farmed Sea Bream (Sparus aurata) Caused By Mycobacterium frederiksbergense in Turkey
Author:
Urku Cigdem1, Genc Gonca Erkose2, Wittwer Franziska3, Erturan Zayre2, Pfyffer Gaby3
Affiliation:
1. Department of Fish Disease, Faculty of Aquatic Sciences , University of Istanbul , 34470, Ordu Cad. No: 200 Laleli- Istanbul / Turkey 2. Department of Medical Microbiology, Istanbul Faculty of Medicine , Istanbul University , Capa, 34093 , Istanbul / Turkey 3. Department of Medical Microbiology , Luzerner Kantonsspital, 6000 Luzern 16, Switzerland
Abstract
Abstract
Over the past decades, different mycobacteriosis agents have become important fish pathogens. The present study describes a different mycobacteriosis in farmed sea bream (Sparus aurata) caused by Mycobacterium frederiksbergense in Turkey. Affected 15 fish, weighing 15 to 20 g, showed lethargy, stunted growth, pale skin, dorsal fin necrosis, and a significant level of mortality (40 %) in fish stocks. Internally, no multifocal white-colored granulomas in the visceral organs were observed. Inoculation of the visceral organs onto Löwenstein-Jensen medium and Tryptic Soy Agar (1.5% NaCl) slants produced only fast-growing (2-3 weeks), orange to yellow-colored, photochromogenic acid-fast colonies. Ziehl-Nielsen positive bacterial isolates were identified using a commercially available line probe assay (Genotype Mycobacterium CM/AS assay) and with 16S rRNA gene sequencing analysis based on 16S rRNA gene sequencing, fifteen isolates were identified as Mycobacterium frederiksbergense. Histopathologically, epithelioid cell granulomas were not observed in any vicseral organs but acid-fast bacteria were detected in the liver, kidney, spleen, and heart tissue. This study shows that asystemic mycobacteriosis is observed in sea bream with high mortality.
Publisher
Walter de Gruyter GmbH
Subject
General Veterinary
Reference41 articles.
1. 1. Avsever ML, Çavuşoğlu C, Günen MZ, Yazıcıoğlu Ö, Eskiizmirliler S, Didinen BI, Tunalıgil S, Erdal G, Özden M: The first report of Mycobacterium marinum isolated from cultured meagre, Argyrosomus regius. B Eur Assoc Fish Pat 2014, 34: 124-129. 2. 2. Avsever ML, Çavuşoğlu C, Eskiizmirliler S, Türe M, Korun J, Çamkerten İ: First isolation of Mycobacterium marinum from sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus auratus) cultured in Turkey. B Eur Assoc Fish Pat 2016, 36: 193-200. 3. 3. Jacobs JM, Stine CB, Baya AM, Kent ML: A review of mycobacteriosis in marine fish. J Fish Dis 2009, 32: 119–130.10.1111/j.1365-2761.2008.01016.x 4. 4. Kent ML, Whipps CM, Matthews JL, Florio D, Watral V, Bishop-Stewart JK, Poort M, Bermudez L: Mycobacteriosis in zebrafish (Danio rerio) research facilities. Aqua Animal Models of Human Disease 2004, 138: 383-390.10.1016/j.cca.2004.08.005 5. 5. Timur G, Urku C, Canak Ö, Genc Erkose G, Erturan Z: Systemic Mycobacteriosis Caused by Mycobacterium marinum in Farmed Meagre (Argyrosomus regius), in Turkey. Bamidgeh 2015, 67.2015.1162, 1-8.
|
|