Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture-Reference-Cited by-同舟云学术

Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Published:2020-12-01 Issue:4 Volume:8 Page:152-157
ISSN:2544-3577
Container-title:Medical Journal of Cell Biology
language:en
Short-container-title:

Author:

Bryja Artur¹,Pieńkowski Wojciech²,Stefańska Katarzyna³,Chermuła Błażej⁴,Bryl Rut¹,Wieczorkiewicz Maria⁵,Kulus Jakub⁶,Wąsiatycz Grzegorz⁷,Bukowska Dorota⁶,Ratajczak Kornel⁷,Jaśkowski Jędrzej M.⁶,Petitte James N.⁸,Mozdziak Paul⁸⁹,Pawelczyk Leszek⁴,Spaczyński Robert Z.⁴,Antosik Paweł⁶

Affiliation:

1. Department of Anatomy, Poznań University of Medical Science , Poznań , Poland

2. Division of Perinatology and Women’s Diseases, Poznań University of Medical Sciences , Poznań , Poland

3. Department of Histology and Embryology, Poznań University of Medical Science , Poznań , Poland

4. Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical Sciences , Poznań , Poland

5. Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń , Toruń , Poland

6. Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń , Toruń , Poland

7. Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń , Toruń , Poland

8. Prestage Department of Poultry Science, North Carolina State University , Raleigh, NC , USA

9. Physiology Graduate Program, North Carolina State University , Raleigh, NC , USA

Abstract

Abstract The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture. These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells. Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone. Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique. Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed. Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells

Publisher

Walter de Gruyter GmbH

Subject

Cell Biology,Molecular Biology

Link

https://www.sciendo.com/pdf/10.2478/acb-2020-0019

Reference25 articles.

1. Xu Y, Niu J, Xi G, Niu X, Wang Y, Guo M, Yangzong Q, Yao Y, Sizhu SL, Tian J. TGF-β1 resulting in differential microRNA expression in bovine granulosa cells. Gene. 2018;663:88–100; DOI:10.1016/j.gene.2018.04.036.

2. Corduk N, Abban G, Yildirim B, Sarioglu-Buke A. The effect of vitamin D on expression of TGF β1 in ovary. Exp Clin Endocrinol Diabetes. 2012;120:490–3; DOI:10.1055/s-0032-1314858.

3. Nagashima T, Kim J, Li Q, Lydon JP, DeMayo FJ, Lyons KM, Matzuk MM. Connective tissue growth factor is required for normal follicle development and ovulation. Mol Endocrinol. 2011;25:1740–59; DOI:2019041122541184000.

4. Piotrowska H, Kempisty B, Sosinska P, Ciesiolka S, Bukowska D, Antosik P, Rybska M, Brüssow KP, Nowicki M, Zabel M. The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review. Vet Med. 2013;58:505–15; DOI:10.17221/7082-VETMED.

5. Kranc W, Budna J, Kahan R, Chachuła A, Bryja A, Ciesiółka S, Borys S, Antosik P, Bukowska D, Brüssow KP, Bruska M, Nowicki M, Zabel M, Kempisty B. Molecular basis of growth, proliferation, and differentiation of mammalian follicular granulosa cells. J Biol Regul Homeost Agents. 2017;31:1–8.

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Human Granulosa Cells—Stemness Properties, Molecular Cross-Talk and Follicular Angiogenesis;Cells;2021-06-05